TOPO-FLAG-GATA4
| Backbone | pCR-BluntII-TOPO |
|---|---|
| Insert | FLAG-mGATA4 |
| Seq | cDNA / amino acid |
| Site | EcoRI/XhoI |
| Selection | Kan |
Insertion
TOPO...M13 Fwd→...EcoRI-EcoRI-FLAG-GATA4-XhoI→-EcoRI...←M13 Rev...TOPO
Note
FLAG-GATA4 cDNA was amplified by using pcDNA-FLAG-GATA4 as a template.