TOPO-GATA1
| Backbone | pCR-BluntII-TOPO |
|---|---|
| Insert | mGATA1 |
| Seq | cDNA / amino acid |
| Site | BamHI/XhoI |
| Selection | Kan |
Insertion
TOPO...M13 Rev→...EcoRI-BamHI-GATA1-XhoI→-EcoRI...←M13 Fwd...TOPO
Note
GATA1 cDNA was amplified by using mouse bone marrow cDNA as a template.