TOPO-GATA2
| Backbone | pCR-BluntII-TOPO |
|---|---|
| Insert | mGATA2 |
| Seq | cDNA / amino acid |
| Site | EcoRI/XhoI |
| Selection | Kan |
Insertion
TOPO...M13 Fwd→...EcoRI-EcoRI-GATA2-XhoI→-EcoRI...←M13 Rev...TOPO
Note
GATA2 cDNA was amplified by using mouse bone marrow cDNA as a template.