TOPO-GATA3
| Backbone | pCR-BluntII-TOPO |
|---|---|
| Insert | mGATA3 |
| Seq | cDNA / amino acid |
| Site | EcoRI/NotI |
| Selection | Kan |
Insertion
TOPO...M13 Fwd→...EcoRI-EcoRI-GATA3-NotI→-EcoRI...←M13 Rev...TOPO
Note
GATA3 cDNA was amplified by using mouse bone marrow cDNA as a template.