TOPO-GATA4-3'UTR

Backbone	pCR-BluntII-TOPO
Insert	mGATA4-3'UTR
Seq	cDNA
Site	XbaI (GATA4-3'UTR has a XbaI site)
Selection	Kan

Insertion

TOPO...M13 Fwd→...EcoRI-XbaI-GATA4-3'UTR-XbaI→-EcoRI...←M13 Rev...TOPO

Note

GATA4-3'UTR cDNA was amplified by using mouse heart cDNA as a template. GATA4-3'UTR has a XbaI site. Use TOPO-GATA4-3'UTR-SpeI for inserting GATA4-3'UTR into XbaI site.