TOPO-GATA4-3'UTR-SpeI
| Backbone | pCR-BluntII-TOPO |
|---|---|
| Insert | mGATA4-3'UTR |
| Seq | cDNA |
| Site | SpeI |
| Selection | Kan |
Insertion
TOPO...M13 Fwd→...EcoRI-SpeI-GATA4-3'UTR-SpeI→-EcoRI...←M13 Rev...TOPO
Note
GATA4-3'UTR cDNA was amplified by using mouse heart cDNA as a template. GATA4-3'UTR has a XbaI site. SpeI can be ligated to XbaI.