TOPO-GATA5
| Backbone | pCR-BluntII-TOPO |
|---|---|
| Insert | mGATA5(M383L) |
| Seq | cDNA / amino acid |
| Site | EcoRI/XhoI |
| Selection | Kan |
Insertion
TOPO...M13 Fwd→...EcoRI-EcoRI-GATA5(M383L)-XhoI→-EcoRI...←M13 Rev...TOPO
Note
GATA5 cDNA was amplified by using pGATA5 plasmid from Hasegawa lab as a template. pGATA5 has a polymorphism M383L (A1147C), which is corresponded to rat GATA5. The region including this amino acid is not conserved in human.