Index
Custom primer
| Gene | Seq (5'-3') | bp | Note | Reference |
|---|---|---|---|---|
| Acta1 α-actin |
CGTCACCAGGGTGTCATGG CAGGATACCTCGCTTGCT |
81 | Skm | |
| Actb β-actin |
GTCGTACCACAGGCATTGTGATGG GCAATGCCTGGGTGCATGGTGG |
493 | Internal control |
|
| Alpl ALP |
CAGGGTACACCATGATCTCACC CGCCCATACCATCTCCCAGG |
201 | Osteocyte | Tatsumi, 2007 |
| Ascl1 Ascl1/Mash1 |
GAGAACGAGTTGGGGACG CTTTGATCGTGCTTCGCA |
69 | bHLH Neuron |
Marro, 2011 |
| Ascl4 Ascl4/Mash4 |
GCCGCCATCAGCTACATCAAGCAAC TGCTTTGGACTCGCCATCGCTGTT |
84 | bHLH SC |
|
| Cald1 Caldesmon 1 |
AAGAATCCTTGGGACAGGTGACAG GGATCAAACTCCTTCTGACGCTCT |
202 | SM | |
| Ccna1 Cyclin A1 |
CGCTTGCTAATCGCCCAGACAGAG CAGTCAACACCCCTAGCACGGTTC |
203 | ||
| Ccnd1 Cyclin D1 |
CCAGAGGCGGATGAGAACAAGCAG CAGGAGAGGAAGTTGTTGGGGCTG |
173 | ||
| Cdh15 M-cadherin |
AGTGTGTCTGAGAACCACAAACGC CCAAGTCCAAGGCAAAGGCCCTTA |
226 | SC | |
| Cdk9 Cdk9 |
ACGGCCTCTACTACATCCACA GCTACGAGTCCACATCTCTGC |
275 | Takaya, 2009 | |
| Cdkn1a p21Waf1 |
CTTTGACTTCGTCACGGAGACGCC GAATCTTCAGGCCGCTCAGACACC |
228 | ||
| Cdkn1b p27Kip1 |
CGCCCGAGGAGGAAGATGTCAAAC GCCTGTAGTAGAACTCGGGCAAGC |
286 | ||
| Cdkn1c p57Kip2 |
CAGGAGCCGTCCATCACCAATCAG GTTGAAGTCCCAGCGGTTCTGGTC |
235 | ||
| Cnn1 Calponin 1 |
GGCCTCAAAGACGGGATCATTCTT GTGTTTTCAAACAGGTCGTTGGCC |
188 | SM | |
| Col2a1 Collagen II |
AAGTCACTGAACAACCAGATTGAGA AAGTGCGAGCAGGGTTCTTG |
70 | Chondrocyte | Alexandrovich, 2008 |
| Ep300 p300 |
CTACAGCAGGGCTCTCCTCAG GGTGAACCATAAGGACTGGGATT |
101 | Kaichi, 2010 | |
| Fabp4 aP2 |
GTGATGCCTTTGTGGGAACCTGGAAG TCATAAACTCTTGTGGAAGTCACGCC |
385 | Adipocyte | Takanabe, 2008 |
| Flt4 VEGFR3 |
TGGTACCGGCTCAACCTCTC CACGTTTTTGCAGTCCAGCA |
72 | Lymphatic | Kreuger, 2006 |
| Gapdh GAPDH |
TTGCCATCAACGACCCCTTC TTGTCATGGATGACCTTGGC |
409 | Internal control |
|
| Gata1 GATA1 |
CCTGCGGCCTCTATTTCAA GATTCGACCCCCGCTTCTTTTTC |
120 | ||
| Gata2 GATA2 |
TCACCCCTAAGCAGAGAAGC CCCACAGGCATTGCACAGGTAG |
128 | ||
| Gata3 GATA3 |
GCAGAACCGGCCCCTTATCAA ACCGGGTCCCCATTAGCGTTCCT |
131 | ||
| Gata4 GATA4 |
TCTCACTATGGGCACAGCAG GCGATGTCTGAGTGACAGGA |
136 | ○: Intact Gata4 ○: Truncated Gata4loxP |
Kaichi, 2010 |
| Gata4Wt GATA4 |
TTAAGCCTCAGCGCCGCCTGT GTCTTAGATTTATTCAGGTTC |
226 | ○: Intact Gata4 —: Truncated Gata4loxP |
|
| Gata5 GATA5 |
TTTGAAGGCAGAGTCCAGTC AGGCAAAGTCTTCAGGTTCG |
134 | Di Lisi, 2007 | |
| Gata6 GATA6 |
GACGGCACCGGTCATTACC ACAGTTGGCACAGGACAGTCC |
132 | ||
| Hes1 Hes1 |
AAGATAGCTCCCGGCATTCCAAGC ACCTCGTTCATGCACTCGCTGAAG |
163 | bHLH | |
| Hes3 Hes3 |
CTCTGATGGAGAAGAAGCGCCGTG GTCCTTGCAGTGAGTTCTGGAGGC |
171 | bHLH | |
| Hes6 Hes6 |
CAACGAGAGTCTTCAGGAGCTGCG GCATGCACTGGATGTAGCCAGCAG |
191 | bHLH | |
| Hey1 Hesr1 |
CCACCATGAAGAGAGCTCACCCAG GATGCCTCTCCGTCTTTTCCTGGC |
173 | bHLH | |
| Hey2 Hesr2 |
CAGGCTACAGGGGGTAAAGGCTAC CTGAGATGAGAGACAAGGCGCACG |
164 | bHLH | |
| Heyl Hesr3 |
GGACCCATCGATGTGGGTCAAGAG GGAGGAGCCCTGTTTCTCAAAGGC |
195 | bHLH | |
| Id1 Id1 |
CTGAACGGCGAGATCAGTGCCTTG GCTGCGGTAGTGTCTTTCCCAGAG |
179 | bHLH | |
| Id2 Id2 |
CTGACCACCCTGAACACGGACATC CGTGTTCTCCTGGTGAAATGGCTG |
218 | bHLH | |
| Id3 Id3 |
GCGCACTGTTTGCTGCTTTAGGTG CGCGAGTAGCAGTGGTTCATGTCG |
208 | bHLH | |
| Id4 Id4 |
CTGGAGACTCACCCTGCTTTGCTG CTCAGCGGCAGAGAATGCTGTCAC |
168 | bHLH | |
| Ihh Indian hedgehog |
CCACCTTCAGTGATGTGCTTATTT CGATGACCTGGAAAGCTCTCA |
69 | Chondrocyte | Alexandrovich, 2008 |
| Kdr Flk1/VEGFR2 |
TCTGTGGTTCTGCGTGGAGA GTATCATTTCCAACCACCC |
270 | VEC | Ogawa, 2006 |
| Lyve1 LYVE1 |
TGCTCCCTCCAGCCAAAA AATGCAGGAGTTAACCCAGGTG |
70 | Lymphatic | Kreuger, 2006 |
| Map2 Map2 |
CATCGCCAGCCTCGGAACAAACAG TGCGCAAATGGAACTGGAGGCAAC |
262 | Neuron | |
| Meox1 Meox1 |
ACTCAGAAGATAGCAGCGCCTCAG AAGTACCATGCAATCCTCCCCCAA |
329 | Mesoderm | |
| Mesp2 Mesp2 |
ATCCCTGGGTGACACCTCCTTATT GGGTTCTGGAGACACAGAAAGACT |
218 | bHLH Mesoderm |
|
| Msgn1 Mesogenin 1 |
ATGGACAACCTGGGTGAGACCTTC TGCTGTAATCCAGCTCGACTAAGC |
271 | bHLH Mesoderm |
|
| Myh1 MHC2x |
TGGAGGACCAAATCATCAGC CAGAAGCCAGTTTCCCTGTG |
135 | Skm | Motohashi, 2013 |
| Myh2 MHC2a |
TGACAACTCGTCTCGCTTTG AAGTGACCCGGGACTTCTCT |
110 | Skm | Motohashi, 2013 |
| Myh3 MHC3 |
CACCTGGAGAGGATGAAGAAGAA AAGACTTGACTTTCACTTGGAGTTTATC |
301 | Skm | |
| Myh6 α-MHC/MHC6 |
GGAAGAGTGAGCGGCCATCAAGG CTGCTGGAGAGGTTATTCCTCG |
302 | CM | |
| Myh7 β-MHC/MHC7 |
ATGCTGACAGATCGGGAGAA GGTTGGCTTGGATGATTTGA |
181 | CM/Skm | |
| Myh7b β-MHCb/MHC7b |
GGCACCCTTGAGGATCAAAT GCTATCAATGTCCGCAGAGG |
156 | CM/Skm | |
| Myl1 MLC3f |
CAGTTCATTCTCCAGTCCCGCTGC ATTTGTGGGATTGGTGCCCAGAGC |
180 | Skm | |
| Myod1 MyoD |
GATGGCATGATGGATTACAGCGGC GTGGAGATGCGCTCCACTATGCTG |
161 | bHLH Skm |
|
| Myog Myogenin |
CCCTATTTCTACCAGGAGCCCCAC GCGCAGGATCTCCACTTTAGGCAG |
348 | bHLH Skm |
Watanabe, 2011 |
| Nefh NF200 |
AGTGGTTCCGAGTGAGGTTGG TGGTAGGAGGCAATGTCTGCC |
211 | Neuron | |
| Neurod1 NeuroD1 |
GCTCCAGGGTTATGAGATCGTCAC CCTCGTCCTGAGAACTGAGACACTC |
155 | bHLH Neuron |
|
| Nkx2-5 Nkx2.5 |
CAAGTGCTCTCCTGCTTTCC GGCTTTGTCCAGCTCCACT |
136 | CM | Hidaka, 2003 |
| Otx1 Otx1 |
AGGCGCTGTTCGCAAAGA CCTCCTCGCGCATGAAGAT |
51 | Neuron | |
| Pax7 Pax7 |
CGCGTCCAGGTCTGGTTCAGTAAC GTACTGTGCTGCCTCCATCTTGGG |
193 | SC/MB | |
| Pdgfra CD140a/PDGFRα |
AGGTCTTTCTGGTCCTCAGCTGTC GTTAACCACTTCCAGCACTGTGAC |
251 | Lateral/paraxial mesoderm |
|
| Pdpn gp38/Podoplanin |
CAGTGTTGTTCTGGGTTTTGG TGGGGTCACAATATCATCTTCA |
92 | Osteocyte | Tatsumi, 2007 |
| Pecam1 CD31/PECAM1 |
GTCATGGCCATGGTCGAGTA AGCAGGACAGGTCCAACAAC |
169 | VEC | Ogawa, 2006 |
| Phex Phex |
GTGCATCTACCAACCAGATACG TCTGTTCCCCAAAAGAAAGG |
67 | Osteocyte | Tatsumi, 2007 |
| Pou3f2 Brn2 |
CGCGCATTTGACAGTTACTT GAGAGAGAGTGGGAGAAGCG |
73 | Neuron | Marro, 2011 |
| Pparg PPARγ |
CCCACCAACTTCGGAATCAG TGCTGGAGAAATCAACTGTGGTA |
100 | Adipocyte | Takanabe, 2008 |
| Prox1 Prox1 |
GTTCCACAGACCAGACGGAAG CAGAGGCAGATTGCTCGGAT |
69 | Lymphatic | Kreuger, 2006 |
| Pthlh PTHrP |
CTTGCGCCGCCGTTT TCTGATTTCGGCTGTGTGGAT |
61 | Chondrocyte | Alexandrovich, 2008 |
| Rn18s 18S rRNA |
CGCACGGCCGGTACAGTGAAACTG CACCCGTGGTCACCATGGTAGGCA |
344 | Internal control |
Hirai, 2010 |
| Slc17a7 V-glut1 |
GTGCAATGACCAAGCACAAG TAGTGCACCAGGGAGGCTAT |
70 | Neuron | Marro, 2011 |
| Sost Sclerostin |
CTTCAGGAATGATGCCACAGAGGT ATCTTTGGCGTCATAGGGATGGTG |
133 | Osteocyte | Tatsumi, 2007 |
| Sox9 Sox9 |
CGGCTCCAGCAAGAACAAG TGCGCCCACACCATGA |
63 | Chondrocyte | Alexandrovich, 2008 |
| Syn1 Syapsin 1 |
GGAAGGGATCACATTATTGAGG TGCTTGTCTTCATCCTGGTG |
74 | Neuron | Marro, 2011 |
| Tagln SM22α/Transgelin |
TTCCAGGTGTGGCTGAAGAATGGT CACTGCTGCCATATCCTTACCTTC |
225 | SM | |
| Tcf15 Meso1 |
AAGCTGTCTAAGATCGAGACGCTG TACACCCCTCACTTTCAAGCAGCT |
249 | bHLH Mesoderm |
|
| Tcf21 Tcf21 |
CAAGGCCTTCTCCAGGCTCAAGAC GTTGGCGGTCACCACTTCCTTCAG |
226 | bHLH | |
| Tmem8c Myomaker |
ATCGCTACCAAGAGGCGTT CACAGCACAGACAAACCAGG |
107 | Skm | Millay, 2013 |
| Tpm1 Tropomyosin 1a |
AGAAGGCTGCAGATGAGAGTGAGA TCGCTCTCGATGATGACCAGCTTA |
175 | SM | |
| Tubb3 Tuj1 |
GCGCATCAGCGTATACTACAA TTCCAAGTCCACCAGAATGG |
73 | Neuron | Marro, 2011 |
| Vcam1 VCAM1 |
TCTTGGGAGCCTCAACGGTACTTT TTCGCGTTTAGTGGGCTGTCTATC |
187 | VEC | Marro, 2011 |
| Zfpm1 FOG1 |
GGAGACATGTCCAGGAGGAAAC CCATGGCCTTGGCTTCTTC |
91 | Kozhemyakina, 2014 | |
| Zfpm2 FOG2 |
AGCCATTCAGACAAACCAGG CATCTCTCTGAAACACTTCTAGCTCTC |
85 | Kozhemyakina, 2014 |
Designed primer
| Gene | Company | Cat No | bp | Note | Reference |
|---|---|---|---|---|---|
| Afp AFP |
Integrated DNA Technologies |
Mm.PT.58.32760839 | Ex1-3 120 |
||
| Hes2 Hes2 |
Integrated DNA Technologies |
Mm.PT.58.42961537 | Ex1-3 125 |
bHLH | |
| Hes5 Hes5 |
Integrated DNA Technologies |
Mm.PT.58.33573251.g | Ex1-2 111 |
bHLH | |
| Hes7 Hes7 |
Integrated DNA Technologies |
Mm.PT.58.23529930 | Ex1-3 140 |
bHLH | |
| Heyl Hesr3/Hey3 |
Integrated DNA Technologies |
Mm.PT.58.10119443 | Ex1-3 102 |
bHLH | |
| Mesp1 Mesp1 |
Integrated DNA Technologies |
Mm.PT.58.42064685.g | Ex1-2 92 |
bHLH Mesoderm |
|
| Msc Musculin/MyoR |
Integrated DNA Technologies |
Mm.PT.58.9980480 | Ex1-2 114 |
bHLH Skm |
|
| Myf5 Myf5 |
Integrated DNA Technologies |
Mm.PT.58.5271235 | Ex1-3 106 |
bHLH Skm |
|
| Myl2 MLC2v |
Integrated DNA Technologies |
Mm.PT.58.23343307 | Ex1-3 139 |
CM/Skm | |
| Pax3 Pax3 |
Integrated DNA Technologies |
Mm.PT.58.13416818 | Ex1-2 126 |
||
| T brachyury |
Integrated DNA Technologies |
Mm.PT.58.17138722.g | Ex1-2 112 |
||
| Tcf15 Meso1/Praxis |
Integrated DNA Technologies |
Mm.PT.58.9453848 | Ex1-2 146 |
bHLH Mesoderm |
|
| Tcf23 Tcf23 |
Integrated DNA Technologies |
Mm.PT.58.43483473 | Ex1-2 139 |
bHLH |