DNA ligation
Material
- Sol I in DNA Ligation Kit (TaKaRa 6022, -20C)
Protocol
| Reagent | Vol |
|---|---|
| Backbone plasmid | 1.5 ul |
| Insert cDNA | 3.5 ul |
| TaKaRa sol I | 5 ul |
| Total | 10 ul |
- Make mixture shown in table.
- Incubate at RT more than 30 min.
- Transform mixture into DH5a.
See Bacterial transformation with plasmid.