TOPO cloning
Material
- Zero Blunt TOPO PCR Cloning Kit (Invitrogen 450245, -20C)
Protocol
| Reagent | Vol |
|---|---|
| pCR-BluntII-TOPO | 0.5 ul |
| PCR product | 0.5 ul |
| Salt sol | 0.5 ul |
| H2O | 1.5 ul |
| Total | 3.0 ul |
- Make mixture shown in table.
- Incubate at RT more than 30 min.
- Transform mixture into DH5a.
See Bacterial transformation with plasmid.